An in vivo microscopy dataset for the characterization of leukocyte cell death.

Recent advancements in intravital microscopy have enabled the study of cell death in vivo under a variety of experimental conditions, such as infection and cancer. However, the limited throughput of this technology, together with a lack of openly accessible datasets, affects the development of algorithms for the automatic detection and characterization of cell death, which would require the integration of extensive and curated datasets. To address these needs, we present a curated dataset of microscopy videos acquired within the spleen and lymph node of mice under inflammatory conditions associated with the death of neutrophils, eosinophils, and dendritic cells. The dataset provides time-lapse imaging data, the coordinates in space and time of cell death events displaying apoptotic–like morphodynamics, along with the 3D reconstruction of the cell morphology at each time point. Altogether, these data will be pivotal for the development of computer vision and bioimage analysis methods to advance cell death research.

Please find below the links to access imaging data, metadata, experimental details, and annotations for each video of the collection